7.003 | Spring 2022 | Undergraduate

Applied Molecular Biology Lab

Experiment 1, Lab Day 4

Parts Pages in Lab Manual
Part 1.8: Identifying α-factor-resistant candidates 34
Part 1.9: Streaking α-factor-resistant candidates onto new plates 34–36

Day 4 In-Lab Questions (PDF)

Pre-Lab and Post-Lab Instructions

Day 4 Pre-Lab Tips and Reminders

Remember to include Aims for Parts 1.8 and 1.9.

Day 4 Post-Lab Tips and Reminders

  • Record the number of α-factor-resistant colonies on each replica-plate.
  • Record which library pool each of your final selected six α-factor-resistant mutants originated from. (Why do you think this might be important to know?)

Course Info

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As Taught In
Spring 2022
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Labs
Activity Assignments
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Written Assignments