| Parts | Pages in Lab Manual |
|---|---|
| Part 3.3: Complete genomic DNA prep from pRSQ2 transformants | 58–59 |
| Part 3.4: Restriction enzyme digest of the genomic DNA | 60–61 |
| Part 3.5: Pour agarose gel | 61–62 |
Pre-Lab and Post-Lab Instructions
Day 9 Pre-Lab Tips and Reminders
Include Aims for all parts (Parts 3.3–3.5).
Day 9 Post-Lab Tips and Reminders
- Record all spectrophotometer measurements/results.
- Remember to include the calculations for setting up your digest reactions.
- How successful do you think your overall genomic DNA prep was, based on your spectrophotometer results? What can you conclude from the A260/A280 ratios?
