Lab Day 14
- Part 4.1: Isolation of total RNA from WT and mutant yeast
- Part 4.2: Pour agarose gel
Lab Day 15
- Part 4.3: Gel analysis of isolated RNA
- Part 4.4: Preparation of cDNA from RNA
Lab Day 16
- Part 4.5: Perform FUS1 test PCR with cDNA
- Part 4.6: Pour agarose gel
Lab Day 17
- Part 4.7: Gel analysis of cDNA FUS1 PCR test samples
- Part 4.8: Preparation of FUS1 qPCR samples
Lab Day 18
- Part 4.9: Analysis of the FUS1 qPCR results