7.003 | Spring 2022 | Undergraduate

Applied Molecular Biology Lab

Experiment 4: Analyzing RNA Expression of α-Factor Resistant Mutants

Lab Day 14

  • Part 4.1: Isolation of total RNA from WT and mutant yeast
  • Part 4.2: Pour agarose gel

Lab Day 15

  • Part 4.3: Gel analysis of isolated RNA
  • Part 4.4: Preparation of cDNA from RNA

Lab Day 16

  • Part 4.5: Perform FUS1 test PCR with cDNA
  • Part 4.6: Pour agarose gel

Lab Day 17

  • Part 4.7: Gel analysis of cDNA FUS1 PCR test samples
  • Part 4.8: Preparation of FUS1 qPCR samples

Lab Day 18

  • Part 4.9: Analysis of the FUS1 qPCR results

Course Info

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Spring 2022
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