LOCUS       pHIS8_4_Lucifera        6996 bp ds-DNA     circular     19-FEB-2015
DEFINITION  Complementary copy of pET-28a(+).
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|532134159|
COMMENT     VNTDBDATE|532134296|
COMMENT     VNTNAME|pHIS8-3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             2920..2944
                     /dnas_title="lac operator"
                     /vntifkey="4"
                     /label=lac operator
                     /ApEinfo_fwdcolor=#ffa249
                     /ApEinfo_revcolor=#4f86ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4925..5380
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          3051..4703
                     /organism="Photinus pyralis"
                     /mol_type="mRNA"
                     /db_xref="taxon:7054"
                     /country="USA: Illinoi, Palos Forest"
                     /collection_date="27-May-2008"
                     /collected_by="Yuichi Oba"
                     /identified_by="Marc A. Branham"
                     /label=source:Photinus pyralis
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(5476..6288)
                     /dnas_title="kan sequence"
                     /vntifkey="4"
                     /label=kan sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      1..1
                     /dnas_title="ColE1 pBR322 origin"
                     /vntifkey="33"
                     /label=ColE1 pBR322 origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1435..2514)
                     /dnas_title="lac I"
                     /vntifkey="4"
                     /label=lac I
                     /ApEinfo_fwdcolor=#ff8d89
                     /ApEinfo_revcolor=#ff728a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3051..4703
                     /gene="PpyLuc1"
                     /codon_start=1
                     /product="firefly luciferase"
                     /protein_id="BAL46512.1"
                     /db_xref="GI:374081834"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
                     /label=firefly luciferase
                     /ApEinfo_fwdcolor=#ccff00
                     /ApEinfo_revcolor=#b7ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      4842..4888
                     /dnas_title="T7 terminator"
                     /vntifkey="43"
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#ff1ece
                     /ApEinfo_revcolor=#34ff2b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    4757..4774
                     /dnas_title="His tag"
                     /vntifkey="31"
                     /label=His tag
                     /ApEinfo_fwdcolor=#1db0ff
                     /ApEinfo_revcolor=#ff2d37
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2901..2917
                     /dnas_title="T7 promoter"
                     /vntifkey="30"
                     /label=T7 promoter
                     /ApEinfo_fwdcolor=#f9ff4a
                     /ApEinfo_revcolor=#5769ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             2975..2980
                     /dnas_title="rbs"
                     /vntifkey="32"
                     /label=ribosomal binding site (rbs)
                     /ApEinfo_fwdcolor=#5dff56
                     /ApEinfo_revcolor=#ff5ff3
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2994..3017
                     /dnas_title="N-His tag"
                     /vntifkey="21"
                     /label=N-His tag
                     /ApEinfo_fwdcolor=#43c1ff
                     /ApEinfo_revcolor=#ff6561
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2988..2990
                     /note="Start Codon"
                     /label=Start Codon
                     /ApEinfo_fwdcolor=#39ff33
                     /ApEinfo_revcolor=#ff48ea
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3024..3044
                     /note="TE Protease Site"
                     /label=TE Protease Site
                     /ApEinfo_fwdcolor=#7fffb5
                     /ApEinfo_revcolor=#ff65f5
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3044..3049
                     /note="NcoI Diagnostic Digest Site"
                     /label=NcoI Diagnostic Digest Site
                     /ApEinfo_fwdcolor=#ff000a
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      181 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      241 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
      301 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
      361 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
      421 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
      481 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
      541 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
      601 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
      661 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
      721 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
      781 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
      841 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
      901 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
      961 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     1021 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     1081 cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     1141 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     1201 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     1261 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     1321 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     1381 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     1441 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1501 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     1561 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     1621 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     1681 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     1741 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     1801 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     1861 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     1921 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     1981 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     2041 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     2101 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     2161 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     2221 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     2281 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     2341 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     2401 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     2461 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     2521 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
     2581 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
     2641 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     2701 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     2761 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     2821 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
     2881 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
     2941 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg aaacaccacc
     3001 accaccacca ccaccacggt ggtGAAAACT TGTACTTCCA GGCCcatggc atggaagacg
     3061 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     3121 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     3181 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     3241 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     3301 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     3361 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     3421 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     3481 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     3541 tgtacacgtt cgtcacgtct catctacctc ccggttttaa tgaatacgat tttgtaccag
     3601 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctggg tctactgggt
     3661 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     3721 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     3781 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     3841 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     3901 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     3961 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     4021 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     4081 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     4141 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     4201 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     4261 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     4321 cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     4381 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     4441 tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg
     4501 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     4561 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     4621 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     4681 agggcggaaa gtccaaattg taaccatggc ggatccgaat tcgagctccg tcgacaagct
     4741 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
     4801 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     4861 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
     4921 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5341 tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5461 atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     5521 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     5581 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     5641 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     5701 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
     5761 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     5821 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     5881 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     5941 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     6001 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     6061 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     6121 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     6181 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     6241 atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     6301 attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt
     6361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6541 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6961 ttttgtgatg ctcgtcaggg gggcggagcc tatgga  
//
